Bioanalytical techniques (CH861)
Programme:
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Credits (L-T-P):
Content:
Basics of analytical chemistry; Qualitative and quantitative analysis of proteins, nucleic acids, polysaccharides and small molecules such as antibiotics, vitamins, natural products etc. Spectroscopic methods-UV, Vis, IR, Fluorescence, ORD, CD, & PAS), Chromatography: HPLC (including ELSD, RI and UVD detectors), GC, Ion chromatography; Mass spectrometry:atomic absorption spectrometry, atomic emission spectrometry, Recent developments in applications to proteomics and metabolomics (SELDI, MALDI, Q-TOF, Triple Quad and Ion trap mass analyzers), SEM, TEM and their application in bioprocessing and bioproduct characterizations. Introduction to methods and concepts of statistical analysis, errors, precision, accuracy, analysis of variance, confidence interval.